Anti-Histone H3 (asymmetric di methyl R17) Antibody

From Antibodies.com, this rabbit polyclonal IgG antibody targets Histone H3 bearing asymmetric dimethylation at arginine 17 (R17/Arg17), a 17 kDa protein. The unconjugated reagent is raised in rabbit and has been validated for reactivity with human, mouse, and rat samples.

According to the manufacturer's datasheet, the antibody is validated for western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), and immunoprecipitation (IP), with recommended dilutions of 1:500–1:1,000 for WB and 1:50–1:200 for ICC/IF and IP. The Antibodies.com product listing additionally lists immunohistochemistry (IHC) among the supported applications.

The immunogen is a synthetic asymmetric dimethylated peptide around R17 of human histone H3 (NP_003520.1), with the sequence APRKQ. The antibody is affinity purified and supplied as a liquid in phosphate buffered saline (pH 7.3) containing 50% glycerol and 0.05% Proclin 300. It is shipped at 4°C and should be aliquoted and stored at -20°C upon delivery, with freeze/thaw cycles to be avoided.