Anti-Histone H3 Antibody

Supplied by Antibodies.com as an unconjugated rabbit polyclonal reagent, this Histone H3 antibody is a primary antibody packaged in a 50 µl vial. The target protein, histone H3, sits at the core of the nucleosome and is a central marker in chromatin biology; its post-translational modification states (such as methylation at H3K4, H3K9, and H3K27 and acetylation at H3K9/K14) are routinely profiled in gene regulation, DNA damage response, and cell differentiation studies.

Validated in Western blot and ELISA applications, the reagent recognizes endogenous histone H3 in human, mouse, and rat samples, supporting its use both as a normalization control in whole-cell and nuclear lysates and as an input anchor for chromatin immunoprecipitation workflows. The unconjugated format offers flexibility to pair the primary with a laboratory-selected secondary antibody, choosing HRP, biotin, or fluorophore reporters based on the detection chemistry, while the polyclonal nature broadens epitope coverage on the H3 tail.

Handle as a temperature-sensitive protein reagent: refrigerate on arrival, aliquot into smaller working volumes to limit freeze-thaw cycles, and protect from light once a reporter conjugate has been added. For research use only.