Anti-Histone H3 Antibody

The Antibodies.com rabbit polyclonal antibody recognizes Histone H3, a core component of the nucleosome that organizes eukaryotic DNA into chromatin. Used in epigenetics, cancer biology, and gene regulation research, the unconjugated reagent detects endogenous Histone H3 in lysates and tissue samples from human, mouse, and rat origins.

The antibody is validated for Western blot and ELISA workflows, supporting studies of post-translational histone marks, chromatin remodeling, and nucleosome composition. Western blot applications commonly pair the primary antibody with HRP-linked secondary reagents to visualize histone bands in acid-extracted nuclear fractions. ELISA-based assays exploit antibody recognition of native histone epitopes for quantitative profiling of histone content across experimental conditions.

Each vial delivers 100 µL of liquid polyclonal antiserum; aliquoting is recommended to minimize freeze-thaw cycles and preserve activity. Store at –20 °C for long-term stability, with working stocks kept at 4 °C. The product is intended for research use only in laboratory settings.