Anti-Histone H3 Antibody

This rabbit polyclonal antibody from Antibodies.com targets Histone H3, one of the four core histones (H2A, H2B, H3, H4) that package eukaryotic DNA into nucleosomes. Because Histone H3 is constitutively expressed and highly conserved across mammals, antibodies raised against it serve two common roles in the lab: as a nuclear-localization marker in immunofluorescence, and as a loading control for normalizing Western blot signals between lanes.

The reagent is supplied as 100 µl of unconjugated antiserum and has been validated for Western blot (WB), immunofluorescence (IF), and ELISA workflows. It cross-reacts with Human, Mouse, and Rat samples, supporting studies that compare rodent cell lines and human tissue lysates in parallel. On Western blots, a band near 15-17 kDa confirms identity with the native histone, including variants carrying post-translational methylation, acetylation, or phosphorylation marks.

For short-term use, store the vial at 4 °C; aliquot and freeze at -20 °C for extended storage and avoid repeated freeze-thaw cycles. The unconjugated format leaves secondary antibody choice open, so the same primary can be paired with fluorescent, HRP, or alkaline phosphatase reporters depending on the assay.