Anti-Histone H3 Antibody

From the Antibodies.com catalogue, this rabbit polyclonal reagent is raised against Histone H3 and is validated for Western blot (WB,1:500–1:1,000), immunohistochemistry (IHC, 1:50–1:100) and ELISA (1:1,000). The immunogen is a synthetic peptide derived from the N-terminal region (amino acids 1–50) of human Histone H3, and the antibody is affinity-purified from rabbit serum on the immunizing peptide, yielding an IgG isotype in unconjugated format. It shows broad reactivity with Human, Mouse and Rat samples and is supplied as a 50 µl liquid aliquot intended for research use only. Store at −20 °C on receipt to preserve activity, avoiding repeated freeze/thaw cycles. The reagent is well suited as a total-Histone H3 loading control in chromatin and nuclear-extract studies, and as a partner in epigenetic and gene-expression workflows where pan-H3 detection is required alongside modification-specific antibodies.