Anti-Histone H3 Antibody

This rabbit-derived polyclonal reagent from Antibodies.com targets Histone H3, a highly conserved core nucleosomal protein that packages genomic DNA into chromatin and serves as a substrate for diverse post-translational modifications regulating transcription, DNA repair, and cell-cycle progression. Supplied as 100 µl of unconjugated antiserum, the antibody supports multiple research workflows including Western blot detection of histone extracts, immunohistochemistry of tissue sections, immunofluorescence imaging of nuclear staining patterns, immunoprecipitation of native chromatin complexes, and chromatin immunoprecipitation (ChIP) profiling of histone-marked loci. The reagent has been validated for cross-reactivity with Human, Mouse, and Rat samples, making it broadly applicable across comparative cell biology and epigenetics studies. Because Histone H3 expression remains relatively constant across proliferating cell types, it is frequently used as an internal loading control when normalizing chromatin-bound protein inputs. Polyclonal sera raised against this target recognize multiple epitopes, which can be advantageous when probing modified histone variants or denatured lysates.