Anti-Histone H3 (aDi-Methyl R2) Antibody

This rabbit polyclonal antibody from Antibodies.com recognizes Histone H3 when asymmetrically dimethylated at arginine residue 2 (H3R2me2a), an epigenetic modification linked to transcriptional repression at promoter regions and chromatin state changes during cellular differentiation.

The unconjugated reagent is calibrated for detection in Human, Mouse, and Rat samples and is supplied as a 100 µl volume at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, allowing direct incorporation into immunoprecipitation buffers, ChIP workflows, or downstream labeling reactions.

Validation covers multiple research applications: Western blot for acid-extracted histone samples, immunohistochemistry on formalin-fixed tissue sections, immunofluorescence imaging of cultured cells, immunoprecipitation of native nucleosomes, and chromatin immunoprecipitation (ChIP) for sequencing or qPCR analysis of H3R2me2a-enriched genomic loci.

For long-term stability, aliquot upon receipt and store at -20°C, avoiding repeated freeze-thaw cycles to preserve binding activity. The polyclonal response against the asymmetrically dimethylated R2 epitope supports signal strength in low-abundance chromatin pull-downs and underpins studies in cancer epigenetics, embryonic stem cell self-renewal, and reader-writer enzyme characterization.