Anti-Histone H3 (Acetyl-K9) Antibody

Histone H3 acetylated at lysine 9 (H3K9ac) is an epigenetic modification mark associated with transcriptionally active chromatin and is widely used as a marker of open, actively transcribed regions of the genome. Antibodies.com offers a rabbit polyclonal primary antibody raised against this specific acetylated residue, supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2 and packaged as a 100 µl vial.

Validated for Western blot (WB) and immunohistochemistry (IHC) applications, this unconjugated reagent has been tested on samples from human, mouse, and rat, supporting comparative chromatin studies across common model systems in cell biology, developmental biology, and epigenetics research. Because the antibody is unconjugated, the user is free to pair it with chromogenic, fluorescent, or HRP-labelled secondary reagents depending on the chosen experimental readout.

The polyclonal format enables recognition of multiple epitopes surrounding the acetylated K9 residue, which can strengthen signal intensity in western blots of acid-extracted histones and in IHC staining of formalin-fixed, paraffin-embedded tissue sections. For best results, aliquoting on receipt is recommended to limit repeated freeze-thaw cycles of the stock solution. The reagent is intended for research use only.