Anti-Histone H3 (Acetyl-K123) Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against histone H3 acetylated at lysine 123 (Acetyl-K123), a post-translational modification associated with open, transcriptionally active chromatin. The antibody is supplied as 100 µl of liquid formulation at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.3, ready for direct use in Western blotting workflows.

Validated for Western blot detection in human, mouse, and rat samples, the reagent supports comparative studies of histone acetylation across species and tissue types. Researchers investigating epigenetic regulation of gene expression, chromatin remodeling, or the interplay between histone modifications can probe steady-state acetylation levels, evaluate the effects of histone deacetylase (HDAC) inhibitors, or compare modification patterns between wild-type and genetically modified cell lines.

The polyclonal format allows the reagent to recognize multiple epitopes on the acetylated H3 tail, which can strengthen signal in Western blot applications while requiring careful interpretation of modification-specific effects. Sodium azide is included as a preservative; for downstream applications where azide is not desired, the buffer can be exchanged by dialysis or gel filtration before use. Store refrigerated and protect from light to maintain antibody activity across repeated freeze-thaw cycles.