Anti-Histone H3.3B Antibody

The rabbit polyclonal reagent from Antibodies.com targets the histone H3.3B variant, a replication-independent H3.3 isoform encoded by H3-4 and deposited into chromatin outside of S-phase. Supplied as 100 µL of unconjugated antiserum in a single vial, the antibody is validated for western blot, immunohistochemistry, and immunofluorescence workflows on human samples, supporting chromatin biology, epigenetics, and transcription factor recruitment studies. Pairing with H3.3A-specific reagents allows isoform-resolved mapping of H3.3 deposition at active promoters, regulatory enhancers, and pericentromeric heterochromatin. Aliquot upon first thaw to limit freeze-thaw cycles and store refrigerated short-term; for long-term stability, keep frozen at -20 °C protected from light. Researchers should also confirm host-species compatibility with secondary detection reagents, since the polyclonal rabbit background can require additional blocking during IHC and IF staining.