Anti-Histone H3.3 Antibody

Recombinant rabbit monoclonal antibody targeting Histone H3.3, a replication-independent histone H3 variant that deposits at actively transcribed genes, telomeric regions, and pericentromeric chromatin. Antibodies.com supplies this unconjugated primary antibody validated for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry, and chromatin immunoprecipitation workflows across human, mouse, and rat samples. Each vial contains 50 µl of antibody formulated in 1xTBS (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative. The antibody is suited to chromatin biology, epigenetics, transcription regulation, and histone variant deposition studies, including investigation of H3.3 K27M and G34W driver mutations implicated in pediatric diffuse midline gliomas and other solid tumours. Store at 4 degrees C; aliquot to avoid repeated freeze-thaw cycles and protect from light to preserve binding activity. For research use only.