Anti-Histone H3.1 (Ab-10) Antibody - Identical to Thermo (PA5-49579)

Raised in rabbit and affinity-purified by epitope-specific chromatography, this polyclonal reagent from Antibodies.com targets the Histone H3.1 protein and is supplied as an unconjugated, ready-to-use liquid at 1.0 mg/mL in a 50 µL aliquot. The immunogen corresponds to a short peptide spanning residues 8–12 (R-K-S-T-G) of human Histone H3.1, allowing the antiserum to detect endogenous levels of total Histone H3.1 (predicted molecular weight ~17 kDa) in human, mouse, and rat samples. The host species is rabbit (IgG isotype), the clonality is polyclonal, and no fluorophore or enzyme tag is attached, making the reagent suitable for both Western blotting (recommended dilution range 1:500–1:1000) and immunofluorescence applications on cultured cells and tissue sections. Each vial is formulated in phosphate-buffered saline (without Mg²⁺ or Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide as preservative, and 50% glycerol as cryoprotectant; for long-term integrity the product should be stored at –20 °C, while short-term use at 4 °C is acceptable. Validation data on the supplier page include Western blot analysis of 293 cell lysates and representative immunofluorescence images. Identical in sequence to Thermo Fisher product PA5-49579, this antibody is intended for research use only and is not approved for diagnostic or therapeutic applications.