Anti-Histone H3.1 (Ab-10) Antibody - Identical to Thermo (PA5-49579)

Raised in rabbit against the replication-dependent histone variant H3.1, this polyclonal reagent from Antibodies.com detects the canonical H3.1 isoform. Histone H3.1 and the replication-independent variant H3.3 differ at residue 31 (alanine vs. serine) and at positions 87, 89, and 90, allowing isoform-selective recognition by antibodies directed against the Ab-10 epitope region. Supplied as 100 µl of unconjugated antiserum, the product is validated for Western blot and immunofluorescence in human, mouse, and rat samples. Typical applications include profiling nucleosome assembly, mapping epigenetic marks, and examining cell-cycle-coupled histone deposition. The antibody recognizes H3.1 across a range of post-translational modification states, including methylated and acetylated tails, so it serves as a loading reference when probing chromatin-enriched fractions. For handling, store aliquots refrigerated at 4 °C for short-term use and avoid repeated freeze-thaw cycles to preserve binding activity.