Anti-Histone H2B Antibody

Histone H2B forms part of the nucleosome core particle alongside H2A, H3, and H4, packaging genomic DNA into chromatin and serving as a scaffold for post-translational modifications that regulate transcription, DNA repair, and chromatin remodeling. This rabbit polyclonal antibody from Antibodies.com targets an epitope on Histone H2B and supports Western blot, immunohistochemistry, and ELISA workflows across human and mouse samples. Provided as 50 µl of unconjugated antiserum, the polyclonal reagent is suitable for detection of total H2B in nuclear extracts, acid-extracted histone preparations, and whole-cell lysates. Researchers commonly apply H2B antibodies as loading controls when normalizing chromatin-bound protein levels, probing histone ubiquitination patterns, or mapping nucleosome positioning in chromatin immunoprecipitation experiments. The unconjugated format allows flexible conjugation to fluorophores, biotin, or HRP depending on the assay design. Refrigerate upon arrival; aliquot to limit freeze-thaw cycles and preserve antibody activity over multiple experiments.