Anti-Histone H2B Antibody

Histone H2B is one of the core histone proteins that packages DNA into nucleosomes, and this unconjugated rabbit polyclonal reagent from Antibodies.com enables detection of the target across multiple experimental platforms. Raised in rabbit, the antibody is intended for western blot, immunohistochemistry, and ELISA workflows, with reactivity listed for human and mouse samples.

Supplied as 100 µl of unconjugated polyclonal antiserum, the reagent integrates directly into standard immunodetection pipelines without secondary labeling steps. Researchers apply this antibody to chromatin-associated studies, transcriptional regulation investigations, and epigenetic mapping projects where histone modification contexts matter. The polyclonal nature of the reagent increases the chance of recognizing conserved epitopes, useful when working with samples across species boundaries.

Recommended applications include western blot analysis of acid-extracted histone fractions, immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, and ELISA-based quantification of histone H2B levels in cell lysates. Store the vial refrigerated between uses, aliquot to limit freeze-thaw cycles, and protect from light to preserve antibody integrity over extended experimental timelines.