Anti-Histone H2B Antibody

This rabbit polyclonal reagent from Antibodies.com is raised against Histone H2B, a core nucleosomal histone that packages genomic DNA into chromatin and carries post-translational modifications linked to transcription and the DNA damage response. The unconjugated antibody is supplied as 100 µl of polyclonal serum and is validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows, with reactivity confirmed against human and mouse samples. Researchers commonly deploy it to probe H2B expression and modification states in chromatin fractions, to monitor monoubiquitination of H2B (H2Bub1) at Lys120, and to track histone turnover during differentiation, stress responses, or apoptosis studies. As an unconjugated primary reagent, it can be paired with a broad choice of HRP- or fluorophore-labelled secondary antibodies, making it adaptable to chromogenic and fluorescence-based detection platforms. For storage, refrigerate the vial on receipt and aliquot to limit freeze-thaw cycles, particularly important for chromatin IP and immunofluorescence applications. The polyclonal format provides broad epitope coverage, which is especially useful when studying modified histone variants or when the target is partially masked by bound chromatin-associated factors.