Anti-Histone H2B Antibody

Histone H2B is one of the four core histones (H2A, H2B, H3, and H4) that assemble into the nucleosome, the fundamental repeating unit of chromatin. This unconjugated rabbit polyclonal antibody from Antibodies.com recognizes endogenous Histone H2B and supports western blot, immunohistochemistry, immunofluorescence, and ELISA workflows in human, mouse, and monkey samples.

The polyclonal format delivers recognition of multiple epitopes across the H2B protein, including regions that carry post-translational marks such as lysine ubiquitination, serine phosphorylation, and lysine acetylation. Supplied as 100 µl of liquid in a vial, the reagent is unconjugated, allowing researchers to pair it with secondary reporters such as HRP, fluorophores, or biotin for each application.

Applications extend to chromatin fractionation studies, transcription elongation assays, and epigenetic profiling of histone modifications, where detection of total H2B serves as a loading control alongside modification-specific antibodies.