Anti-Histone H2AX Antibody [Assigned #A0823]

This unconjugated mouse monoclonal IgG reagent from Antibodies.com targets Histone H2AX, a nuclear histone H2A variant encoded by the H2AFX gene that is rapidly phosphorylated at Ser139 by ATM and ATR kinases to generate γH2AX foci marking DNA double-strand break sites. Researchers studying DNA damage response, chromatin remodeling, and genotoxicity use this antibody to evaluate H2AX expression and modification status in human samples by Western blot (WB) and immunohistochemistry (IHC) on tissue sections and cell preparations. The reagent is supplied as 100 µl in a vial, with reactivity confirmed specifically for human antigen. Its monoclonal format provides lot-to-lot consistency for reproducible detection across fixed and lysed specimens, making it a practical tool for DDR pathway characterization, oncology research, and radiation biology workflows.