Anti-Histone H2AX Antibody

Manufactured by Antibodies.com, this Rabbit polyclonal reagent is raised against Histone H2AX and detects endogenous levels of the total target protein across validated immunoassays. The immunogen is a synthesized peptide derived from the internal region of human Histone H2AX, and the antibody is affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen to maximize specificity. Supplied as an unconjugated, ready-to-use liquid at 1 mg/ml, the formulation consists of Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, with 150 mM NaCl, 0.02% sodium azide and 50% glycerol as a cryoprotectant, and the product should be stored at −20 °C to preserve activity. This 50 µl pack size is validated for Western blot (WB) and immunofluorescence (ICC/IF) applications, and it has been confirmed to react with Human samples, making it a reliable tool for DNA-damage response and chromatin research workflows, including studies of γH2AX foci formation following double-strand breaks. Known synonyms for the target include H2A.X, H2a/x, H2AFX and H2AX, and the antibody is shipped on blue ice from Cambridge, UK, with a typical lead time of 4–6 business days. For research use only; not intended for diagnostic or therapeutic applications.