Anti-Histone H2AFX (phospho S2) Antibody

Raised in rabbit and affinity-purified, this unconjugated polyclonal IgG selectively binds histone H2AFX (also referred to as H2AX) only when serine 2 is phosphorylated, enabling researchers to probe this post-translational modification by Western blot on human and rat samples. The 100 µl format ships as a liquid aliquot that should be stored refrigerated and protected from repeated freeze-thaw cycles to preserve phospho-epitope reactivity. Validated application coverage is restricted to Western blot, and the unconjugated preparation is compatible with secondary antibody-based chemiluminescent workflows run on whole-cell lysates and nuclear extracts. This reagent from Antibodies.com targets a chromatin-associated histone mark used in studies of DNA damage signaling, nucleosome remodeling, and cell-cycle checkpoint regulation where phosphorylation status at H2AFX serine 2 is the variable of interest.