Anti-Histone H2A.X (phospho-S139) Antibody

This unconjugated mouse monoclonal antibody from Antibodies.com selectively recognises Histone H2A.X when phosphorylated at serine 139, the modification widely cited as gamma-H2AX and used as an early readout of DNA double-strand break formation. The reagent is supplied as 100 µl of purified IgG in 0.1 M Tris-Glycine buffer at pH 7.4, containing 150 mM NaCl, 0.2% sodium azide, and 50% glycerol.

Validated for Western blot and immunofluorescence workflows, the antibody is reactive with both human and mouse samples, supporting comparative studies across cell lines, primary cultures, and tissue sections from either species. In immunofluorescence, the antibody highlights discrete nuclear foci that assemble at sites of chromatin damage following genotoxic insults, replication stress, or exposure to ionising radiation. Western blot analysis resolves a band near 15 kDa, allowing researchers to track phosphorylation kinetics across treatment time courses.

Working aliquots of the glycerol stock should be refrigerated to limit repeated freeze-thaw cycles and protect reagent activity, and the vial should be shielded from light during storage. The product is intended for research use only, supporting applications in DNA damage response, radiobiology, cancer research, and chromatin biology.