Anti-Histone H2A.X (E145) Antibody

This Rabbit polyclonal reagent from Antibodies.com is raised against Histone H2A.X and is directed at the E145 epitope within the protein. It is supplied as a 50 µl liquid formulation at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide, adjusted to approximately pH 7.2, and is presented unconjugated for use as a primary antibody. The host species is Rabbit, the clonality is polyclonal, and reactivity has been validated in Human, Mouse and Rat samples, making it suitable for a broad range of experimental models. The recommended application is immunohistochemistry (IHC), where it can be used to detect endogenous levels of Histone H2A.X (also referred to by the synonyms H2AFX, H2AX, H2a/x and Histone H2AX). As supplied by Antibodies.com, the antibody is affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen, providing high specificity for the E145 region of Histone H2A.X. Store the vial according to standard short-term storage at 2–8 °C, avoiding repeated freeze–thaw cycles to preserve activity. For IHC, users typically titrate the antibody within the 1:50–1:200 range, depending on tissue type and detection system. This reagent is intended for research use only and is not approved for diagnostic or in vivo applications.