Anti-Histone H2A.X Antibody

Raised in rabbit and affinity-purified by epitope-specific immunogen chromatography, this unconjugated polyclonal reagent from Antibodies.com is directed against endogenous Histone H2A.X and is supplied as a 50 µl liquid formulation at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody is of the IgG isotype and was generated against a synthesized peptide derived from the human Histone H2A.X sequence flanking the non-phosphorylated Ser139 residue, allowing recognition of the H2AX/H2AFX histone variant across human, mouse and rat samples. It is validated for Western blot, immunohistochemistry, immunocytochemistry/immunofluorescence and ELISA workflows, making it suitable for chromatin, DNA-damage response and nuclear marker studies in cell and tissue models. The product is shipped at 4 °C and should be aliquoted upon arrival and stored at −20 °C, with freeze/thaw cycles avoided to preserve performance. Researchers seeking a broadly reactive anti-H2A.X reagent for multi-application use will find this Antibodies.com polyclonal a flexible addition to the lab, with a 100 µl pack size also available for higher-throughput needs.