Anti-Histone H2A.X Antibody

Phosphorylation of histone H2A.X at Ser139 generates γH2AX, one of the earliest chromatin marks installed at DNA double-strand break sites, and this recombinant rabbit monoclonal reagent from Antibodies.com is raised specifically against that phospho-S139 epitope on Histone H2A.X. The unconjugated antibody is supplied as 50 µl of recombinant monoclonal antibody in TBS (pH7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, a formulation that tolerates standard -20°C storage and limited freeze-thaw cycling without loss of binding activity.

It is validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human, mouse, and rat samples, supporting nuclear γH2AX foci analysis in fixed tissue sections and phosphorylated-H2A.X band detection in whole-cell lysates from DNA damage studies, genotoxicity screens, and cell-cycle checkpoint research. Because the antibody selectively recognizes the S139-phosphorylated form rather than total H2A.X, it enables kinetic monitoring of the DNA damage response following irradiation, topoisomerase inhibition, or replication stress, while the rabbit monoclonal backbone reduces lot-to-lot variability common to polyclonal phospho-specific reagents. Each vial is shipped on blue ice for research use only and is intended for in vitro detection of γH2AX in chromatin biology and radiobiology applications.