Anti-Histone H2A.X (Ab-139) Antibody

Manufactured by Antibodies.com for sensitive detection of the histone variant H2A.X, this Rabbit polyclonal reagent targets an epitope surrounding the well-characterized Ser139 mark of Histone H2A.X. Supplied as a 50 µl aliquot at 1.0 mg/mL in phosphate-buffered saline (pH 7.4, 150 mM NaCl) with 0.02% sodium azide and 50% glycerol as cryoprotectant, the unconjugated preparation is intended for Western blotting, immunohistochemistry, and immunocytochemistry/immunofluorescence workflows on human samples. The antibody was raised in Rabbit against a synthetic peptide spanning residues 137–141 (Q-A-S-Q-E) of human Histone H2A.X and subsequently purified by affinity chromatography on an epitope-specific peptide column, ensuring recognition of the endogenous total Histone H2A.X pool. Reactivity has been validated for Human tissue and cell preparations, while the polyclonal clonality supports a robust signal across applications where epitope accessibility may vary. For routine handling, the reagent is shipped at 4 °C and should be aliquoted upon arrival and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Synonyms annotated for the target include H2a/x, H2AFX, H2AX and Histone H2AX. For research use only.