Anti-Histone H2A.J Antibody

A replication-independent variant histone enriched in senescent cells, Histone H2A.J localizes to senescence-associated heterochromatin foci and serves as a molecular marker for studies of cellular aging, DNA damage response, and chromatin remodeling. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the human Histone H2A.J protein and is validated for Western blot and immunohistochemistry workflows, supporting detection of the endogenous variant in chromatin extracts and fixed tissue sections.

The antibody is supplied as a 100 µg aliquot and should be stored at -20 °C in working aliquots to limit freeze-thaw cycles, with brief bench use kept on ice and protected from light. Because reactivity was confirmed on human samples, researchers probing senescent fibroblasts, aged tissue biopsies, or models of genotoxic stress can use this reagent to track variant incorporation and chromatin redistribution across dividing and arrested cell populations. Pairing it with standard anti-rabbit secondary reagents enables chromogenic or chemiluminescent readouts in WB and IHC, and the unconjugated format leaves the choice of fluorophore, HRP, or biotin label open for downstream assays.