Anti-HIST2H2BE Antibody

This polyclonal antibody from Antibodies.com is raised in rabbit against HIST2H2BE, a replication-dependent histone H2B variant encoded within the histone cluster 2 locus on human chromosome 1q21.2. The reagent is supplied as a 100 µl vial at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant, which lets users dilute it directly into standard blotting and immunostaining buffers. It has been validated for Western blot and immunohistochemistry on human, mouse, and rat samples, supporting chromatin biology and epigenetics work that compares histone incorporation, turnover, or post-translational modification across tissues and species. Because sodium azide is included as a preservative, the antibody should be stored refrigerated and protected from light; for long-term use, small aliquots can be frozen to limit freeze-thaw cycles and preserve binding activity. HIST2H2BE sits in a cluster of nearly identical H2B genes, so this reagent is most informative when paired with matched controls in chromatin fractionation, acid-extracted nuclear preparations, or immunoprecipitation experiments aimed at mapping nucleosome composition. Researchers can apply the unconjugated antibody with standard HRP- or fluorophore-conjugated secondary reagents, or conjugate it themselves for custom multiplex immunoassays.