Anti-HIST1H2AH Antibody

Raised in rabbit against the histone variant encoded by HIST1H2AH, this unconjugated polyclonal antibody recognizes the human H2A type 1-H protein, a core histone that assembles with DNA into nucleosomes and shapes chromatin accessibility throughout the nucleus. The target gene maps within the histone cluster 1 region on chromosome 6p22.2, where closely related H2A paralogs share extensive sequence identity, making well-validated reagents useful for distinguishing specific deposition patterns in chromatin studies.

Supplied by Antibodies.com as a 100 µl liquid aliquot, the reagent is validated for Western blot and immunohistochemistry on human samples. Western blot analysis resolves the histone band at roughly 14 kDa, while IHC on formalin-fixed paraffin-embedded tissue produces nuclear staining consistent with chromatin-associated localization. Researchers studying epigenetic regulation, chromatin remodeling, and histone post-translational modification can use this antibody to confirm expression of the H2A type 1-H variant across cultured cell lines and primary tissues.

The unconjugated format leaves the user free to choose among common secondary reporters and enzyme conjugates, including HRP, alkaline phosphatase, and fluorophore-labeled anti-rabbit IgG, supporting flexibility across Western blot and immunohistochemistry workflows. The 100 µl volume provides enough material for multiple probing experiments and titration optimization, and the polyclonal nature offers broader epitope coverage than monoclonal alternatives for detecting the target across variant and modified contexts.