Anti-His Tag Antibody

This mouse-derived polyclonal reagent from Antibodies.com recognizes the polyhistidine affinity tag commonly engineered onto recombinant proteins. Raised in mouse hosts and supplied as an unconjugated IgG preparation, the antibody is provided in a 50 µl volume and validated for western blot, immunofluorescence, and immunoprecipitation applications.

Researchers rely on this reagent to confirm expression of His-tagged constructs, verify successful purification on immobilized metal affinity chromatography resins, and localize tagged fusion proteins within fixed cells. The polyclonal nature of the antibody supports recognition of multiple epitopes along the polyhistidine motif, providing reliable detection across bacterial, yeast, and mammalian expression systems.

Each preparation is quality-tested to confirm specificity toward the 6xHis tag, helping minimize background in pull-down and co-immunoprecipitation experiments where tag-only controls are essential. For storage, keep refrigerated and aliquot upon arrival to limit freeze-thaw cycles and preserve binding activity for ongoing laboratory workflows.