Anti-His Tag Antibody

A mouse polyclonal reagent raised against the polyhistidine epitope commonly engineered onto recombinant fusion proteins. The unconjugated IgG preparation from Antibodies.com supports Western blot detection of 6xHis-tagged constructs expressed in bacterial, yeast, or mammalian systems, and is equally suited to immunofluorescence staining of fixed cells and immunoprecipitation workflows for co-purification studies. Supplied as a 200 µl vial in liquid format with the standard mouse IgG isotype, this reagent pairs with anti-mouse secondary antibodies labeled with HRP, fluorophores, or biotin depending on the downstream readout. Researchers apply it to confirm expression of tagged proteins after cloning into pET, pBAD, or pcDNA-based vectors, to track purification fractions during immobilized metal affinity chromatography on nickel or cobalt resins, and to localize fusion proteins to specific subcellular compartments. The polyclonal nature broadens recognition across multiple positions within the His-tag stretch, which is helpful when the epitope is partially buried or adjacent to a folded domain. Aliquot upon arrival, store refrigerated, and minimize freeze-thaw cycles to preserve binding activity across repeated experiments.