Anti-His Tag Antibody [10A12]

Mouse monoclonal antibody clone 10A12 from Antibodies.com targets the polyhistidine (6xHis) tag used in recombinant protein expression and purification workflows. Supplied as 100 µg of lyophilised product, this unconjugated IgG1 reagent is validated for Western Blot detection of His-tagged fusion proteins in human cell lysates. The unconjugated format permits flexible pairing with secondary antibodies and custom detection chemistries, while the lyophilised presentation supports long-term storage and reconstitution to a working concentration. Researchers apply clone 10A12 to confirm expression of His-tagged constructs following SDS-PAGE separation and membrane transfer, taking advantage of its reactivity with human samples alongside tagged proteins expressed across other host systems.