Anti-HINT1 Antibody [Assigned #A3912]

Anti-HINT1 monoclonal antibody (#A3912 clone) is a mouse IgG reagent produced by Antibodies.com for research use targeting the histidine triad nucleotide-binding protein 1, a haploinsufficient tumor suppressor and member of the HIT superfamily known for its adenosine diphosphate-ribose hydrolase activity. Validated across western blot, immunohistochemistry, immunofluorescence, and flow cytometry workflows, the unconjugated reagent recognizes human, mouse, and rat HINT1, supporting cross-species studies in oncology, neuroscience, and metabolic signaling from a single lot.

Supplied at 200 µl in a vial format, the monoclonal reagent offers lot-to-lot consistency from a defined IgG backbone and supports detection of endogenous HINT1 expression in cell culture and tissue-derived samples. The recommended storage temperature is 4 °C, and aliquoting helps limit freeze-thaw cycles on repeated access. Recommended applications include western blot for size verification, IHC for tumor and nervous tissue sections, IF for subcellular localization, and FC for cell-surface and intracellular staining panels.

Researchers commonly deploy this antibody to investigate HINT1-mediated tumor suppression, regulation of β-catenin and Wnt signaling, mitochondrial function, and links to neuropathic pain pathways. The unconjugated IgG format allows pairing with HRP, fluorophore, or biotin secondary reagents of choice, providing flexibility across diverse assay designs and detection chemistries in research use only workflows.