Anti-HGF Antibody

This rabbit polyclonal antibody targets Hepatocyte Growth Factor (HGF), a multifunctional cytokine best known for activating the c-MET receptor tyrosine kinase. Manufactured by Antibodies.com as part of their Primary Antibodies range, the reagent is supplied as 100 µl of unconjugated antiserum validated for Western Blot (WB) detection of human, mouse, and rat HGF.

HGF research applications extend across oncology, regenerative biology, and tissue injury studies. Investigators commonly probe HGF expression in hepatocyte and epithelial cell lysates, tumor-derived samples, and conditioned media from mesenchymal cell cultures. The polyclonal format provides recognition of multiple epitopes on the mature alpha-chain and the beta-chain, which can be useful when studying full-length pro-HGF (~92 kDa) alongside its processed forms.

For Western Blot, the antibody is typically paired with HRP-conjugated secondary reagents and chemiluminescent substrates to reveal endogenous HGF in relevant cell lines and tissue homogenates. Store the vial at -20°C in working aliquots to limit freeze-thaw cycles, and protect the preparation from light. Each lot is validated for reactivity against the three listed species, giving laboratories a flexible reagent for comparative studies in human disease models and rodent experimental systems.