Anti-Hexim1 Antibody

Hexamethylene bisacetamide-inducible protein 1 (Hexim1) sequesters the positive transcription elongation factor b (P-TEFb) complex through binding to 7SK snRNA, restraining RNA polymerase II elongation and coupling transcription to physiological signalling cues. This unconjugated rabbit polyclonal reagent from Antibodies.com detects endogenous Hexim1 and is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, delivered as a 100 µl vial. Validation covers western blot, immunohistochemistry, and immunofluorescence on human, mouse, and rat specimens, supporting research into cardiac hypertrophy, estrogen receptor α signalling, and HIV-1 transcriptional reactivation, where Hexim1 displacement from 7SK is the committed regulatory step. Investigators have also applied the antibody to co-immunoprecipitation of native Hexim1–P-TEFb complexes from nuclear extracts and to chromatin immunoprecipitation pilot experiments targeting 7SK-bound chromatin. Store the glycerol stock frozen at -20 °C in working aliquots, keep protected from light, and limit freeze-thaw cycles to preserve binding activity.