Anti-HES5 Antibody

Raised in rabbit against a recombinant fragment of human HES5, this unconjugated polyclonal IgG reagent from Antibodies.com is supplied as a 100 µL vial for Western blot analysis of human and mouse lysates. The antibody recognizes a basic helix-loop-helix transcriptional repressor that operates downstream of Notch receptor activation.

HES5 drives cell fate decisions during neurogenesis, T-cell lineage commitment, and oscillatory segmentation clock dynamics. Researchers use the antibody to characterize Notch-driven progenitor maintenance in developing neural tissue, monitor T-cell differentiation in thymic cultures, and compare transcriptional repressor activity across disease models and knockout controls. The unconjugated format pairs with HRP- or fluorophore-conjugated secondary antibodies matched to rabbit IgG, supporting standard chemiluminescent detection and fluorescent multiplexing on the same blot.

Aliquot on first use and store the vial at -20°C, limiting freeze-thaw cycles to preserve activity. Brief centrifugation before dilution removes any aggregates that accumulate during storage. The polyclonal format tolerates minor sequence variation between the human and murine HES5 orthologs, contributing to consistent signal across cross-species comparative studies. Dilute into TBS-T or PBS-T with skim milk or BSA blocking buffer before probing membranes transferred from Tris-glycine SDS-PAGE gels.