Anti-Hepatitis C Virus NS5a Antibody (FITC) [8926]

This Mouse monoclonal antibody to Hepatitis C Virus NS5A (FITC), supplied by Antibodies.com as a ready-to-use liquid reagent in IgG2a format, is provided at 50 µg per vial.

The fluorescein isothiocyanate conjugation permits direct visualization of the NS5A non-structural phosphoprotein in immunofluorescence microscopy and Western blot workflows without requiring labeled secondary antibodies, simplifying detection of viral protein expression in infected cell cultures.

NS5A is a multifunctional zinc-binding protein that exists in basally phosphorylated p56 and hyperphosphorylated p58 forms, contributing to viral RNA replication and virion assembly within the membranous web. Researchers employ this reagent to track genotype 1b replicon activity, monitor subcellular localization to replication complexes, and characterize antiviral compound effects on the HCV replicase.

For best results, store at -20 °C in single-use aliquots, protected from light to preserve FITC fluorescence, and limit repeated freeze-thaw cycles. The liquid format is ready to be applied directly to fixed cells or transferred to membranes under standard immunofluorescence and Western blot protocols, with reactivity confirmed against Hepatitis C Virus.