Anti-Hepatitis C Virus NS4 Antibody [7E11]

The 7E11 monoclonal antibody from Antibodies.com is a mouse-derived IgG1 reagent raised against the NS4 region of the Hepatitis C Virus (HCV) polyprotein, supplied as a 100 ug lyophilised preparation for reconstitution in laboratory buffer. Produced in mouse hybridoma cells, the unconjugated clone binds native and recombinant NS4 antigen, supporting qualitative detection workflows in hepatitis C virology research. Validated applications include ELISA, immunohistochemistry (IHC), and Western blot (WB), making the reagent compatible with multiple readouts across fixed tissue sections, infected cell lysates, and recombinant antigen capture assays.

Lyophilisation offers stable storage and shipping without cold-chain requirements prior to reconstitution; once rehydrated, the antibody is typically aliquoted to minimise freeze-thaw cycles and preserve binding activity. Because the reagent targets a viral non-structural protein rather than a host antigen, it is used to characterise HCV replicase components, map epitope recognition within NS4, and confirm NS4 expression in transiently transfected cells or subgenomic replicon systems. Researchers working with patient-derived HCV strains can apply the antibody in IHC to localise viral protein within liver biopsy material, while Western blot use enables size verification of NS4 fragments in lysate preparations and ELISA enables serological screening of NS4 reactivity.

Supplied by Antibodies.com as a research-use-only primary antibody, the 7E11 clone provides reproducible specificity for the NS4 target across ELISA, IHC, and WB workflows.