Anti-Hemoglobin (Hb) Antibody

Manufactured by Antibodies.com and distributed through AFMS in the Kingdom of Saudi Arabia, this Goat polyclonal antibody is raised against human Hemoglobin (Hb) and supplied as a 1 mg unit of affinity-purified, lyophilized polyclonal reagent. The unconjugated IgG is validated for ELISA (suggested coating range 1:100–1:500; detection 1:1,000–1:30,000) and Western blotting (1:1,000–1:30,000), and it forms a single precipitin band with adult hemoglobin variants A1, A2, F and S by immunoelectrophoresis, making it well suited to hemoglobinopathy, red-cell and plasma-research workflows. Reconstitution in PBS (pH 7.2) with 0.09–0.1% sodium azide brings the antibody to approximately 1 mg/mL; short-term storage is at 2–8 °C, with aliquots held at –20 °C for long-term use (24-month shelf-life), and freeze/thaw cycles should be avoided to preserve activity. Sold as a research-grade reagent for in-vitro use only, this lyophilized Antibodies.com preparation delivers lot-to-lot consistency for ELISA plates and Western blots probing human Hb in serum, lysates and purified hemoglobin preparations.