Anti-HELLS Antibody

Offered through the Antibodies.com catalogue of primary antibodies, this polyclonal reagent is raised in rabbit against HELLS (also known as LSH, SMARCA6, Nbla10143, or PASG), a chromatin-remodeling factor implicated in DNA methylation and lymphoid development. The antiserum is produced by immunizing rabbits with recombinant human HELLS protein and is subsequently purified by affinity chromatography on the immunogen, yielding an IgG-rich polyclonal preparation. Supplied at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, the antibody is unconjugated and intended for detection of the endogenous total HELLS protein. Validated applications include Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), immunoprecipitation (IP), and chromatin immunoprecipitation (CHIP). The reagent is listed as reactive with human samples and should be stored at –20 °C for long-term stability, with the standard 50 µL pack size offered here as a working aliquot suitable for multiple experiments. Recommended positive controls include HELLS-expressing cell lines such as HeLa, while chromatin extracts prepared from human lymphocytes or HEK293 cells are commonly used to verify performance in CHIP workflows. Researchers studying epigenetic regulation, lymphoid malignancy, or DNA replication-associated genome maintenance will find this affinity-purified polyclonal reagent a versatile tool across orthogonal immunoassay platforms.