Anti-HDGFRP3 Antibody

Raised in rabbit and affinity-purified against the human HDGFRP3 target (also known by the aliases HDGFL3 and HRP-3), this unconjugated polyclonal primary antibody from Antibodies.com recognizes a nuclear member of the hepatoma-derived growth factor family. The PWWP domain within HDGFRP3 drives chromatin association and ties the reagent to studies of transcriptional regulation, hepatocellular carcinoma biology, renal injury models, and stress-response pathways in which altered nuclear protein expression is being characterized.

The product is supplied as 100 µl at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.3, giving laboratories the flexibility to titrate working dilutions against their specific membrane transfer and blocking chemistry. Cross-reactivity has been confirmed for mouse and rat orthologs, so the same vial can be applied to cultured human cell lines alongside rodent tissue samples within a single comparative western blot experiment.

Validated for western blot on whole-cell or nuclear lysates, the antibody pairs with HRP-conjugated or fluorescent secondary reagents. For best results, aliquot upon first thaw to limit repeated freeze-thaw cycles, store refrigerated between uses, and protect from light to preserve the unconjugated protein.