Anti-HDAC8 Antibody

Rabbit polyclonal reagent from Antibodies.com raised against histone deacetylase 8 (HDAC8), supplied as 100 µl of unconjugated liquid in a single vial. HDAC8 is a class I Zn2+-dependent deacetylase that catalyzes removal of acetyl groups from lysine residues on histone and non-histone substrates, contributing to chromatin remodeling, transcriptional regulation, smooth muscle contractility, and cell cycle progression. The antibody is validated for Western blot, immunohistochemistry, and ELISA workflows, and it cross-reacts with human, mouse, rat, and monkey samples, making it practical for human specimen analysis and corresponding rodent model validation. For immunohistochemistry on formalin-fixed paraffin-embedded sections, the reagent yields predominantly nuclear staining consistent with the established subcellular localization of HDAC8, while cytoplasmic signal may be observed in some cell types and tissues. The unconjugated format allows researchers to pair the antibody with HRP, biotin, or fluorescent secondary detection reagents of their choice. The vial should be stored frozen in aliquots to limit freeze-thaw cycles and preserve reactivity over time. Researchers focused on epigenetic regulation, oncology (HDAC8 has been studied in acute myeloid leukemia and neuroblastoma contexts), X-linked intellectual disability linked to HDAC8 mutations, or comparative signal transduction pathways will find this polyclonal reagent a practical addition to their antibody panel.