Anti-HDAC5 Antibody

Raised in rabbit and affinity-purified by epitope-specific immunogen chromatography, this polyclonal reagent from Antibodies.com is designed for the selective detection of endogenous HDAC5 in human, mouse, and rat samples. Supplied as an unconjugated IgG at 1.0 mg/mL, the antibody is formulated in phosphate-buffered saline (pH 7.4, 150 mM NaCl) containing 0.02% sodium azide and 50% glycerol as a cryoprotectant, and should be stored frozen at –20 °C in aliquots to limit freeze–thaw cycles. The 50 µl pack size is validated for multiple immunoassays, including Western blotting, immunohistochemistry, and immunocytochemistry / immunofluorescence, making it suitable for studies of chromatin remodeling, cardiac hypertrophy, and neuronal gene regulation where histone deacetylase 5 plays a well-documented role. The immunogen corresponds to a synthetic peptide derived from the human HDAC5 sequence, allowing the antibody to recognize total endogenous HDAC5 protein across the three tested species without requiring species-specific secondary reagents beyond anti-rabbit conjugates. Because the product is supplied unconjugated, it can be flexibly labeled with fluorophores, biotin, or enzymes depending on the chosen detection workflow, and its polyclonal nature provides broader epitope coverage than monoclonal alternatives—an advantage when probing post-translationally modified or alternatively spliced HDAC5 isoforms. Researchers should note that the antibody is intended for research use only and is not validated for diagnostic or therapeutic applications.