Anti-HDAC4 (N-terminus) Antibody

The antibody recognizes an epitope within the N-terminal regulatory domain of HDAC4, a class IIa histone deacetylase that shuttles between the nucleus and cytoplasm in response to phosphorylation by CaMK and other kinases. This unconjugated mouse monoclonal reagent from Antibodies.com is validated for western blot and immunocytochemistry on human samples, making it useful for studies of chromatin remodeling, transcriptional repression, and signal-dependent gene regulation.

HDAC4 controls skeletal muscle differentiation through MEF2C repression and has been implicated in cardiac stress response, neuronal survival pathways, and several malignancies including medulloblastoma and breast cancer. Researchers use the antibody to track endogenous HDAC4 expression and subcellular localization, including its phosphorylation-driven nuclear export mediated by 14-3-3 binding. As a monoclonal reagent it provides lot-to-lot consistency against a single defined epitope, supporting reproducible detection across independent experiments.

Each vial contains 100 µl of purified immunoglobulin in 0.1M Tris-Glycine buffer (pH 7.4, 150 mM NaCl) supplemented with 0.2% sodium azide and 50% glycerol to preserve antibody activity during refrigerated storage. The reagent is supplied as a single-use vial suitable for downstream western blot membranes and fixed-cell immunocytochemistry preparations, and its human reactivity makes it well suited for translational research into chromatin-modifying enzymes relevant to cancer biology, cardiac hypertrophy, and neurobiology.