Anti-HDAC4 (Ab-632) Antibody

Raised in rabbit against a synthetic peptide spanning a region around residue 632 of human HDAC4, this polyclonal reagent from Antibodies.com recognizes Histone Deacetylase 4, a class IIa deacetylase that shuttles between the nucleus and cytoplasm and modulates transcription through chromatin remodeling and interactions with MEF2-family transcription factors. The unconjugated antibody is supplied as a 100 µl vial and has been validated for Western blot and immunofluorescence detection in human, mouse, and rat samples.

HDAC4 harbors a C-terminal catalytic deacetylase domain and an N-terminal regulatory region enriched in binding sites for transcriptional co-repressors and 14-3-3 proteins; phosphorylation by calcium/calmodulin-dependent protein kinases drives 14-3-3 binding and cytoplasmic retention, linking HDAC4 activity to calcium signaling, muscle differentiation programs, and neuronal survival pathways. Researchers apply this reagent to follow endogenous HDAC4 levels in whole-cell lysates by immunoblotting and to map its subcellular distribution in fixed cells by immunofluorescence, often paired with biochemical fractionation to distinguish nuclear versus cytoplasmic pools under different stimulation conditions. Aliquot upon arrival and store refrigerated to limit freeze-thaw cycles and preserve antibody performance.