Anti-HDAC3 (Y414) Antibody

Histone deacetylase 3 (HDAC3) is a class I deacetylase that operates within NCoR and SMRT corepressor complexes to remodel chromatin and modulate transcription. This rabbit polyclonal antibody from Antibodies.com was raised against HDAC3 carrying a modification at tyrosine 414, allowing selective detection of this post-translationally modified form of the protein in expression studies. The reagent is validated for Western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples, supporting investigations of chromatin remodeling, gene regulation, and signaling pathway analysis.

The unconjugated antibody is supplied as 100 µl at 1 mg/ml in phosphate buffered saline containing 15 mM sodium azide at approximately pH 7.2. Aliquot upon receipt and store at -20 °C to limit freeze-thaw cycles, with working aliquots kept refrigerated during active use. The polyclonal format provides recognition of multiple epitopes on the target, which can improve signal robustness across sample types including cell lysates, fixed tissue sections, and cultured cells for research use only applications in epigenetics, metabolic disease, and immune signaling research.