Anti-HDAC3 Antibody

This monoclonal antibody from Antibodies.com targets Histone Deacetylase 3 (HDAC3), a class I deacetylase enzyme central to chromatin remodeling, transcriptional repression, and cell cycle control. The reagent is raised in mouse hosts and validated for Western blot detection in human, mouse, and rat samples, enabling cross-species investigations in cancer biology, epigenetics, metabolic regulation, and nuclear receptor signaling.

The antibody is supplied as a purified immunoglobulin in 0.1M Tris-Glycine buffer at pH 7.4 with 150 mM NaCl, stabilized with 50% glycerol and preserved with 0.2% sodium azide. Each vial contains 100 µl of unconjugated antibody, allowing direct use in standard immunoblotting workflows without the need for secondary labeling. The monoclonal format ensures batch-to-batch reproducibility and minimizes background variability compared to polyclonal alternatives.

HDAC3 operates as the catalytic core of NCoR/SMRT co-repressor complexes and modulates gene expression by removing acetyl groups from histone and non-histone substrates. Researchers apply this antibody to characterize acetylation-dependent pathways, study differentiation programs, and explore HDAC3 involvement in tumor progression and metabolic disorders. Store aliquots at -20 °C and avoid repeated freeze-thaw cycles to preserve antibody activity. Intended for research use only.