Anti-HDAC2 Antibody

Raised in rabbit, this unconjugated polyclonal antibody from Antibodies.com specifically targets Histone Deacetylase 2 (HDAC2), a class I epigenetic regulator that catalyzes the removal of acetyl groups from lysine residues on histone H3 and H4 tails. Functioning as a core catalytic subunit of multiprotein repressor complexes including NuRD and Sin3, HDAC2 remodels chromatin architecture and silences transcription of genes governing cell cycle progression, differentiation, and apoptotic programs. Researchers apply this reagent across western blot, immunohistochemistry, and immunofluorescence workflows to profile HDAC2 expression and subcellular distribution in human tissue and cell line samples.

HDAC2 has been extensively characterized in oncology research, where its overexpression is documented in lung, breast, colorectal, and gastric carcinomas, and in studies of cardiac hypertrophy and neurodegenerative disease. Conditional knockout studies demonstrate that loss of HDAC2 in neurons enhances memory formation and synaptic plasticity, while sustained upregulation is implicated in Alzheimer's pathology and age-related cognitive decline. The polyclonal format enables recognition of multiple epitopes on the target protein, which is particularly useful when probing post-translational modifications, splice variants, or partially degraded fragments.

Each vial contains 100 ul of liquid antibody, supplied at a working concentration suitable for direct dilution into standard blocking and incubation buffers. For long-term storage, keep the vial frozen at -20 degrees C and divide into smaller aliquots to limit freeze-thaw cycles that can degrade antibody titer over repeated experimental campaigns.