Anti-HDAC10 Antibody

Primary antibodies targeting epigenetic regulators are essential tools for probing chromatin acetylation dynamics and cancer cell signalling. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com recognises HDAC10 (Histone Deacetylase 10), a class IIb zinc-dependent deacetylase that also functions as a polyamine deacetylase (PDAC) with catalytic specificity for N8-acetylspermidine hydrolysis. Raised in rabbit and affinity-purified, the antibody is validated for Western blot detection of the human, mouse, and rat protein, producing a characteristic band near the predicted molecular weight of approximately 70 kDa.

Supplied as a 50 µl vial of liquid formulation, the reagent is recommended for use in standard SDS-PAGE and chemiluminescent Western blot workflows, paired with HRP-conjugated anti-rabbit secondary antibodies for visualisation. Researchers investigating HDAC10 expression in neuroblastoma, gastric carcinoma, non-small cell lung cancer, and cervical cancer models have repeatedly used HDAC10 polyclonal antibodies to correlate tumour protein levels with prognosis, autophagy-mediated drug resistance, and chemoresponse. The antibody is also relevant to studies of KRAS-driven cancer stem-like cell properties and to doxorubicin-sensitisation work, where HDAC10 depletion is known to interrupt autophagic flux and elevate p62/sequestosome-1 accumulation in transformed cells.

Handling guidance: aliquot upon first thaw to limit freeze-thaw cycles and store frozen at -20 °C; the reagent is intended for research use only. Avoid repeated freeze-thaw and protect from prolonged exposure to room temperature to preserve titre.