Anti-HCLS1 Antibody

This polyclonal antibody from Antibodies.com targets HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1), also referred to as HS1, a roughly 75 kDa intracellular signaling protein that serves as a major substrate of the Lyn tyrosine kinase in hematopoietic lineage cells. Raised in rabbit and supplied as an unconjugated reagent in a 100 µl volume, the antibody is validated for Western blot and immunohistochemistry workflows and has been confirmed to react with human, mouse, and rat samples.

HCLS1 is best known for its role in hematopoietic cells, where it couples antigen receptor engagement to actin cytoskeleton remodeling during B cell and T cell activation. Investigators studying lymphocyte signaling, immune synapse formation, cytoskeletal dynamics, and apoptosis regulation in immune cells frequently rely on this reagent. The polyclonal format provides recognition of multiple epitopes on HCLS1, which can be advantageous when probing denatured targets on Western blots or fixed sections in immunohistochemistry.

For Western blot, the antibody works well on whole-cell lysates prepared from lymphoid or myeloid cell lines, with detection of endogenous HCLS1 readily achievable using HRP-conjugated secondary antibodies and chemiluminescent substrates. For immunohistochemistry, antigen retrieval on formalin-fixed, paraffin-embedded sections followed by standard chromogenic detection yields specific cytoplasmic staining in hematopoietic compartments such as spleen and lymph node. The unconjugated format provides flexibility to pair the reagent with a chosen secondary antibody and detection chemistry suited to each experiment. Keep the vial refrigerated; protect from light, and avoid repeated freeze-thaw cycles to preserve activity across the shelf life.