Anti-HCK Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent targets the hematopoietic cell kinase (HCK), a member of the Src family of non-receptor tyrosine kinases implicated in immune cell signaling and myeloid lineage function. The antibody was raised against a recombinant human HCK protein and purified by affinity chromatography using the immunogen, yielding a polyclonal preparation supplied at 1.0 mg/mL in unconjugated form. It is provided in a 50 µL volume as a phosphate-buffered saline formulation at pH 7.4 containing 150 mM sodium chloride, 0.02% sodium azide as preservative, and 50% glycerol for stability. The reagent specifically recognizes the endogenous total HCK protein and has been validated for Western blot (WB) and immunohistochemistry (IHC) workflows. Confirmed reactivity spans human, mouse, and rat samples, making the antibody suitable for cross-species experimental design in studies of hematopoiesis, innate immunity, and kinase signaling pathways. As a polyclonal host-raised reagent in rabbit, the product offers broad epitope coverage of the HCK target, which is particularly valuable for detecting multiple isoforms or post-translational variants of the kinase. Storage at standard short-term refrigerated conditions is appropriate for the glycerol-containing buffer, while longer-term storage at –20 °C preserves antibody integrity. The product is intended for research use only and is not validated or licensed for diagnostic or therapeutic applications, which is consistent with laboratory reagent standards across the Antibodies.com catalog. Researchers evaluating Src-family kinase involvement in leukocyte migration, Fc receptor signaling, or B-cell receptor pathway studies will find this reagent a practical tool for both lysate-based immunoblotting and tissue-section immunohistochemistry.